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one problem in lc-ms
[版面:化学][首篇作者:pennicecream] , 2009年06月09日19:42:05 ,411次阅读,6次回复
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pennicecream
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发信人: pennicecream (飞冰), 信区: Chemistry
标  题: one problem in lc-ms
发信站: BBS 未名空间站 (Tue Jun  9 19:42:05 2009, 美东)

Why I can see baseline seperation using ELSD, while very big tailing peak in
MS. And peaks between two detectors just cannot match. 
what should I do next? help...
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DgniD
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发信人: DgniD (雪佛莱将军), 信区: Chemistry
标  题: Re: one problem in lc-ms
发信站: BBS 未名空间站 (Tue Jun  9 19:49:35 2009, 美东)

Because

These are two different detectors.
MS is more sensitive.

【 在 pennicecream (飞冰) 的大作中提到: 】
: Why I can see baseline seperation using ELSD, while very big tailing peak
in
:  MS. And peaks between two detectors just cannot match. 
: what should I do next? help...



--

※ 来源:·BBS 未名空间站 海外: mitbbs.com 中国: mitbbs.cn·[FROM: 63.196.]

 
skycaptain08
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发信人: skycaptain08 (太尉), 信区: Chemistry
标  题: Re: one problem in lc-ms
发信站: BBS 未名空间站 (Wed Jun 10 02:47:43 2009, 美东)

no necessarily.

ELSD is a generic detector and MS only applies to ionizable compound.  this
is like u r using pda at two different wave length and the profile could be
way different.
【 在 DgniD (雪佛莱将军) 的大作中提到: 】
: Because
: These are two different detectors.
: MS is more sensitive.
: in



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pennicecream
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发信人: pennicecream (飞冰), 信区: Chemistry
标  题: Re: one problem in lc-ms
发信站: BBS 未名空间站 (Wed Jun 10 12:02:18 2009, 美东)

but what i want to know is all of compounds in my sample.
【 在 skycaptain08 (太尉) 的大作中提到: 】
: no necessarily.
: ELSD is a generic detector and MS only applies to ionizable compound. 
this
: is like u r using pda at two different wave length and the profile could
be
: way different.



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dietcigs
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发信人: dietcigs ( 呆死哥), 信区: Chemistry
标  题: Re: one problem in lc-ms
发信站: BBS 未名空间站 (Wed Jun 10 12:41:12 2009, 美东)

check your extra column volume, where most likely the mismatch and
resolution lost came from.

try to match the tubing size and length for both detectors


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dietcigs
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发信人: dietcigs ( 呆死哥), 信区: Chemistry
标  题: Re: one problem in lc-ms
发信站: BBS 未名空间站 (Wed Jun 10 13:06:15 2009, 美东)

check your extra column volume, where most likely the mismatch and
resolution lost came from.

try to match the tubing size and length for both detectors


--

※ 来源:·WWW 未名空间站 海外: mitbbs.com 中国: mitbbs.cn·[FROM: 12.146.]

 
pennicecream
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发信人: pennicecream (飞冰), 信区: Chemistry
标  题: Re: one problem in lc-ms
发信站: BBS 未名空间站 (Thu Jun 11 11:09:31 2009, 美东)

I used the same tube to connect to both detetors. so i do not think that is
problem.
【 在 dietcigs ( 呆死哥) 的大作中提到: 】
: check your extra column volume, where most likely the mismatch and
: resolution lost came from.
: try to match the tubing size and length for both detectors



--

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