发信人: wildfire (飞火), 信区: Chemistry [删除]
标 题: First image of a memory being made (转载)
发信站: BBS 未名空间站 (Sat Jun 27 11:15:30 2009, 北京)
【 以下文字转载自 Biology 讨论区 】
发信人: wildfire (飞火), 信区: Biology
标 题: First image of a memory being made
发信站: BBS 未名空间站 (Sat Jun 27 11:03:55 2009, 北京)
The increase in green fluorescence, right, shows the imaging of protein
synthesis at synapses when memories are made.
The researchers used a fluorescent protein that can change from green to red
when exposed to ultraviolet light. They flashed the cells with light, so
that any proteins that already existed turned red. But when the scientists
induced the cells to form memories, they saw new green proteins appear under
"One distinction between short-term and long-term memory is this requirement
for making new proteins," said co-investigator Wayne Sossin, a
neuroscientist at McGill University in Montreal. "To make it last, you do
need protein synthesis."
The study, conducted by researchers at the Montreal Neurological Institute
and Hospital (The Neuro), McGill University and University of California,
Synapse- and Stimulus-Specific Local Translation During Long-Term Neuronal
Dan Ohtan Wang, Sang Mok Kim, Yali Zhao, Hongik Hwang, Satoru K. Miura,
Wayne S. Sossin, and Kelsey C. Martin
Science 19 June 2009: 1536-1540.
Published online 14 May 2009 [DOI: 10.1126/science.1173205] (in Science
Express Research Articles)
各位高手讨论一下, GFP 到RFP的UV处理转换的效率有多高, 可不可能REVERSE?这个方法追踪新生蛋白是不是足够严格?
※ 修改:·wildfire 於 Jun 27 11:14:24 2009 修改本文·[FROM: 119.36.]
※ 来源:·WWW 未名空间站 中国: mitbbs.cn 海外: mitbbs.com·[FROM: 119.36.]